CO2 Stage Calibration

  1. Move the laser to the center hole.
  2. Select the correct stage map e.g 221-hole
  3. Select pychron-auto as the calibration style
  4. Hit Calibrate

Pychron will now automatically find up to five calibration holes. The calibration holes are specified on the third line of the stage map file e.g 221-hole.txt. The calibration holes should be the N,E,S,W, and center holes.

Using the calibration holes Pychron calculates the center position and rotation of the tray. With an accurate calibration, Pychron will then move to each hole and determine a corrected position. This will take a few minutes.

Autofocus

Pychron has an auto focus feature that can produce a very sharp image. Configuration allows you to use various alogrithms to calculate the focus measure of an image. I find the Laplace filter with ~50% zoom produces a nice result. Autofocus is actual a misnomer in this case. What is really happenig is called passive focus. The focus measure is calculated by applying a mathematical filter the the image. These filters are used for example to calculate the gradient between adjacent pixels. Theoretically maximizing the gradient yields the most focused image. For more information see Autofocus

Hit Autofocus to perform an autofocus routine